Narishige Pc10 Manual New [better] -

Proper installation is critical to ensure safety and prevent environmental factors from interfering with pipette reproducibility. Environmental Requirements Place the unit on a heavy, vibration-free laboratory bench.

Securely tighten the terminal screws and recalibrate your Melting Reference Point . 6. Troubleshooting Guide Probable Cause Corrective Action Heater setting is too low or weights are too light.

Once the first-stage drop distance is reached, a microswitch engages. The heater setting adjusts (either increasing or decreasing based on configuration) and the main pull weights drop rapidly, snapping the glass waist to create two matching, microscopic tips. 4. Step-by-Step Operating Instructions Step 1: Initial Calibration and Inspection

The process stops after an initial "softening" pull and then performs a second, more intense pull. This is essential for producing firm microelectrodes with the sharp tips (0.1µm to several µm) required for patch clamp experiments. 3. Setting Your Parameters narishige pc10 manual new

The of your glass capillaries (e.g., 1.5mm OD, 0.86mm ID)

Narishige PC-10 Glass Pipette Puller: Setup, Operation, and Maintenance Manual

Secure the top clamp first, ensuring the capillary is perfectly vertical. Proper installation is critical to ensure safety and

The Narishige PC-10 isn’t just a puller; it’s a time capsule of mechanical genius. Before digital touchscreens and software updates, the PC-10 used gravity, a magnetic solenoid, and a carefully calibrated heater to pull glass capillaries into fine-tipped micropipettes. No firmware crashes. No DRM. Just physics and skill.

Understanding the physical layout and control interface is necessary before executing a pull. Physical Components

The Narishige PC-10 is a joystick-operated micromanipulator that allows users to control the movement of a microscope's stage or other instruments with incredible precision. Its ergonomic design and intuitive controls make it easy to use for extended periods, reducing fatigue and increasing productivity. The heater setting adjusts (either increasing or decreasing

Select the desired number of . Adding weights creates shorter, stiffer tapers with larger tip diameters; removing weights yields longer, flexible tapers with smaller tip openings. Step 4: Initiating the Pull Turn on the main power switch.

The PC-10 is compatible with various glass capillaries (e.g., borosilicate, aluminosilicate).

Ensure the vertical tracks sit perfectly perpendicular to the benchtop.

Use a damp cloth to clean the rod and ensure the movement is smooth. Do not use oils or lubricants on the main shaft. Conclusion